ROBoCoP: a Respiratory disease Occupational Biomonitoring Collaborative Project
M Rinaldo, J Pralong, N Hopf, JJ Sauvain, G Suarez, P Wild, I Guseva Canu,
Institute for Work and Health, University of Lausanne, University of Geneva, Switzerland
Chronic obstructive pulmonary disease (COPD) is a major public health problem with a prevalence in Switzerland reaching 15.9% in men. Exposure to particulate matter (PM) is a known COPD risk factor via oxidative stress pathway. Reactive oxygen species (ROS) and their metabolites could be predictive to COPD onset and be used as diagnostic biomarkers.
ROBoCop project, in the framework of CliniMark EU-COST Action, aims to select and validate a panel of oxidative stress biomarkers analyzed in non-invasive biological samples.
The research is divided into three studies:
- An experimental study aiming to standardize the biological sampling procedures and analytic techniques for oxidative stress biomarkers (8-isoprostane, malondialdehyde, NO2-, NO3-, formate ions and 8-OHdG) in exhaled breath condensate and urine and for oxidative potential in the exhaled air (OPEA) analyzed with our OPEA analyzer (EU and USA patent) a promising method in COPD diagnosis.
- A pilot study in the field, at a Parisian Transport Company (RATP) to determine the best time to collect samples and assess the suitability of the selected biomarkers in surveying RATP workers exposed to PM.
- A field study conducted among 300 RATP workers selected by stratified randomization from 45’000 workers. Statistical analyzes of medical examination (lung function test) and biomarkers data will aim to determine the associations of one or a panel of the selected biomarkers with obstructive syndrome (FEV1/FVC<70%).
Our results will allow us to validate a useful and cost-effective method in screening large populations at risk of COPD. It will also be useful in monitoring COPD evolution and severity in patients.
ROBoCop project is supported by the Swiss National Scientific Foundation and French RATP company and involves researchers and occupational physicians from Switzerland and France.
(Posted on 11 January 2018)